The corrected absorbance is within 5% of the. The SpectraMax190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. 00 . Exceptional performance The advanced optical and electronicExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. A = 2 - log 10 (%T). 5%T at 220 nm and 340 nm using NaNO2 Absorbance range. 0 nm increments . SPECTRAmax® 190. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. SpectraMax® M-Series Readers, SpectraMax® Paradigm® Systems, FlexStation® 3 Systems. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both the microplate format and assay signal stability. 006 O. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 8% trisodium citrate aqueous solution. We don't know when or if this item will be back in stock. We enable science by offering product choice, services, process excellence and our people make it happen. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. Synthesis and crystallization of complex 1. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. The SpectraMax 190 Microplate Reader's optical system is built around a monochromator, allowing the selection of up to six wavelengths at a time for absorbance detection in the UV-Vis wavelength range (190 nm - 850 nm). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Cell Apoptosis Assay Following treatment, the apoptosis rate of H9c2 cells was analyzed by flow cytometry analyses using the Annexin V-FITC/PI Cell Apoptosis Detection Kit (Dojindo. Offer Ends In: 9 Days 19: 59: 40. 0 out of 5 $ 4,499-5%. SoftMax® Pro Software User Guide — 0112-0140 Rev. 078 μg/mL), and the results were expressed as μg Fe (II)/g of extract. DNA provides the same sensitivity as a spectrophotometer. The Spectramax 190 has. Plus 384 - SpectraMax Plus 384 Microplate Reader - SpectraMax Plus 384 Absorbance Plate Reader, Molecular Devices - Each . SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. 315 can't find zero order. 118 Gemini XPS Fluorescence Microplate Reader . Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. Wavelength Range: 190 - 850 nm. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Add to cart. The ELISA signal was measured using the ELISA plate reader SpectraMax 190 (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers. 2 - 3. Plasma biomarker levels were measured by enzyme-linked immunosorbent assay (ELISA; R&D unless otherwise stated) using a SpectraMax 190 plate reader (SpectraMax 190 microplate reader, RRID : SCR_018932). 009-QG. 2 nm * Wavelength Range 190 – 850 nm * Photometric. Absorbance was acquired at 450 nM in the microplate reader SpectraMax 190. Compounds were tested for inhibition of 3CL pro following a similar protocol with several small modifications. What is the part number for the filter wheel motor? I am looking to replace mine because I suspect it is the cause of the failure• SpectraMax® 190 (Serial#-NNR-xxxxx) •ersaMax™ (Serial#-BNR-xxxxx) V • AquaMax® Microplate Washer. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Each sample has a discrete sample beam and reference beam so that each well is measured directly. ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. 96/384-well plate formats. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. When the user applies a set temperature of 37°C, the top is heated to 37. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Molecular Devices Spectramax 190 Microplate Reader Molecular Devices Spectramax 190 Microplate Reader Specifications. Capable of detecting DNA down to 25 ng. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The blood was centrifuged at 3000 rpm for 15. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Glow-type luminescence provides an extended signal time window enabling batch processing of plates in screening assays;Colorimetric changes were measured using a SpectraMax 190 microplate spectrophotometer at 450 nm with a wavelength correction set at 620 nm. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Introduction. S. SPECTRAmax 190 Microplate Spectrophotometer Operator’s Manual 2-3 Chapter 2 Setting Up for Stand-Alone Use 1) Place the SPECTRAmax 190 on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. no. D 1 1. Robust, high-value microplate readers that can run samples. . Microplate Spectrophotometer. The data are shown in optical density (O. 81. Microplate spectrophotometer (54 pages) Measuring Instruments Molecular Devices FlexStation II Operator's Manual. The minimum detection limit of the assay was 18. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Open the catalog to page 1. See the installation guide or user guide. TFC values were expressed as rutin equivalents (mg) per gram DW. 0200-0729. SpectraMax® 340PC. The . A 5 mL of aqueous solution of VOSO 4 (0. 2. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-The SPECTRAmax 190 is equipped with an 8-pin DIN RS-232 serial port through which a computer can communicate with and control the instrument. Bioz Stars score: 98/100, based on 1 PubMed citations. 1. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190. When connecting for the first time to either the SpectraMax L reader or StakMax system. SpectraMax® Paradigm® Multi-Mode Detection Platform. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Ltd. It measures the optical density (OD) of samples in 96-wellLow-volume, high-throughput DNA and protein detection on the SpectraMax ABS Plus Microplate Reader. 00 shipping. Prepare your i3/i3x instrument for shipment. Example: [Drawer]. 05,. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The multi-channel design truly mimics a dual-beam spectrophotometer. 1 in stock. The dual-scanning capability can also be used to determine excitation and emission settings for new fluorescent probes. SpectraMax®iD3Multi-ModeMicroplateReader SpectraMax®iD5Multi-ModeMicroplateReader SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader. Electrical: 100-240V, 43/63Hz, 4 A, Used Molecular Devices SPECTRAmax 190 Microplate Spectrophotometer for sale. The . Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. Wavelength Selection . SpectraMax 190 Absorbance Microplate Readerwith laptop and softwareEnables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the. Service Contracts, Preventative Maintenance and Performance. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Molecular Devices Spectrophotometer Spectra MAX 190. 7°C and the bottom to 36. Rose Bengal is a photoreactive compound with its absorbance peaks at 258 nm, 519 nm, and 562 nm Citation 33. . TheSpectraMax® SpectraMax 190 (Serial number starting with N) SpectraMax 250 SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. A mixture of DPPH (0. Simplify and expedite experiments with SoftMax Pro Software’s. It measures the optical density (OD) of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength. . The absorbance of the reaction mixture was recorded at 593 nm (SpectraMax 190, Molecular devices) after 5 min. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive measurementsofavarietyofanalytesacrossawiderangeofconcentrations. ®iD5. SpectraDrop High-Throughput Screening (HTS) Kit. Soluble PA fractions (20 μL) were mixed with 100 μL of DMACA reagent on a 96-well plate. This technical note is designed to provide information . The MIC was determined by the concentration where there was an obvious clear-cut decrease in the absorbance. Thông Số Kỹ Thuật Máy Quang Phổ Định Lượng DNA/RNA SpectraMax Quickdrop - Khoảng bước sóng: 190 -1100 nm - Thể tích nhỏ nhất: 0. Near bottom. Fluorescent Polarization: Solid black plates, as above. 9000-0756. Offer Ends In: 38 Days 16: 07: 54. Three-dimensional Matrigel culture. Upgradeable modules are also available including western blot, cell imaging, and fast. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Description 1. The 293T cells were seeded into 6-well plates, using 330,000 cells. 15 mmol). We would like to show you a description here but the site won’t allow us. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. Description 1. * dsDNA sensitivity specified for the following instruments: SpectraMax® 190 Readers, SpectraMax® Plus384 Readers,. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Microplate Readers Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. Luminescence. Fluorescence and fluorescence polarization. 30% phenol. Select the Driver tab and click Update Driver. For very concentrated samples, use a minimum volumeSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 17247 MOLECULAR DEVICES MICROPLATE SPECTROPHOTOMETER SPECTRAMAX 190 quantity. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 0 out of 5 $ 9,995 $ 9,495. 0 nm. 7. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Brand: Molecular Devices. DNA standards of known concentrations ranging from 1. (SpectraMax ® 190, Molecular Devices). . Absorbance equation. (190 nm - 1000 nm). The . 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13SpectraMax 190 Absorbance Microplate Reader. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. The VersaMax and SpectraMax 190 read 96-well microplates. The standard curve was made using iron (II) sulphate solution (40–0. Small volumes (5 μL, 50 mM) of compound solution, dissolved into DMSO, were added to the. Not all manufacturers microplates are the same with regard to design, mate- rials, or conÞguration. The analysis was performed in triplicate and the results were expressed as mg gallic acid equivalent per g of extract (mg GAE/g). 314 low lamp level warning. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 00 +$350. Streamline GLP/GMP compliance. 1) Select the correct plate and the wells that you want to measure. See SpectraMax® Microplate Reader User Manual Download Page. Spectrophotometric assays were carried out using a SpectraMax 190 Microplate Reader (96-well flat bottom cell culture plate—Kasvi®). For A Higher Degree Of Automation, The Automation Vendor. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Wavelength Accuracy: < ± 2. These models can no longer be repaired. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. The SpectraMax 190 reader is the tool for DNA analysis. The mixture was shaken and incubated for 30 min at room temperature in the dark. . It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The multi-channel design truly mimics a dual-beam spe. Monochromator, tunable 1. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. Year Built: N/A. Wavelength Accuracy: < ± 2. For very concentrated samples, use a minimum…200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaksIMI detection was carried out using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of imidacloprid residue in leaf tissue and using a spectrophotometer (SpectraMax 190; Molecular Devices, San Jose, CA, USA). Auction Details Bio + Laboratory Equipment Expo. 6 stars - 1134 reviewsSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. It measures the optical density (OD) of samples in 96-wellPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. ABTS Determination. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Microplate Spectrophotometer Operator’s Manual. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. The VersaMax and SpectraMax 190 read 96-well microplates. A microplate reader for endpoint, kinetic, and spectral scan assays at UV-visible wavelengths, with temperature control, shaking capabilities, and multichannel optical design. 1 in stock. #730. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- StakMax Handling Stacker Base Unit. The temperature in the plate chamber is ambient and isothermal. edu) to be added as a trained user in iLab. 5mm filters. NO assayIMI detection was conducted using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of IMI residues in leaf tissue and using a spectrophotometer (SpectraMax 190, Molecular Devices, San Jose, CA, USA). SpectraMax® 340PC384. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. After establishing a stable baseline, reactions were started by the addition of 1 µM La-dependent MDH purified as described before ( 41 ). The protein concentration was measured by an enhanced BCA protein assay kit (Beyotime; Nanjing, China). For more sample throughput, the system can The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. Biotek PowerWave XS Microplate Reader BioTek Synergy HTX Microplate Reader - Absorbance Only BioTek ELx808 Ultra Microplate Reader - Absorbance OnlySpectraMax Plus or SpectraMax 190 and SoftMax Pro Software automatically: •Make A 260 and A 280 measurements •Measure the optical pathlength of each sample with the PathCheck sensor •Normalize the absorbance values to a 1 cm pathlength •Calculate concentrations •Make specialized calculations (e. e. Molecular Devices’ PathCheck® sensor technology. 1. Part # 0112-0085 Rev. SpectraMax 190酶标仪适用于大多数生命科学研究工作，尤其是DNA分析。能够检测任何标准96孔或UV-透射96孔微孔板。内置单色器，连续波长范围为190-850nm,步进波长1nm。提供终点检测、动力学和光谱扫描三种测读模式。具有温控系统和微孔板震荡混. For customers outside the U. After incubation, the unbound planktonic cells (120 μl) were. Simplify and expedite experiments with SoftMax Pro. #3635 or Greiner cat. The plate was mixed on an orbital shaker for 15 s and immediately read using a SpectraMax iD3 (Molecular Devices; San Jose, CA, USA) and an integration time of 400 ms. Fluorescence Microplate Reader Comparison Chart. Type: Clear: Sale. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. Manufacturer: Molecular Devices; Model: SpectraMax 190 * Wavelength Selection Monochromator, tunable 1. 7 and HaCaT cells were pretreated with MAEO at 12. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Highly selective for dsDNA over ssDNA or RNA. Plus 384 Protocol (MS Word) For more. Wavelength bandwidth ≤ 2. We have 1 Molecular Devices SpectraMax 190 manual available for free PDF download: User Manual . Italic and boldface type are used for emphasis. Here, we demonstrate several different ways the ABS Plus reader, in combination with the SpectraDrop™ Micro-Volume Microplate and SoftMax® Pro Software, can quantitate double-stranded DNA and bovine serum albumin (BSA. . 000 (OD). The inter- and intra-assay CV for the SAA analysis were less than 10%. Temperature control and shaking come as standard. 0 nm full width half maximum. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Wavelength range 190–1000 nm Wavelength selection Monochromator, tunable in 1. Minimize sample volume while retaining sensitivity with the SpectraDrop Micro-Volume Microplate. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. : HY-30237 and HY-16578). (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 190 – 1100 nm Wavelength accuracy : ±2 nm Stray light <0. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Optical density was measured at 570 nm with an automatic microplate spectrophotometer (SpectraMax 190, Sunnyvale, USA). T&Cs & Important Info. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. For more detailed information and technical support of Molecular Devices instruments or software,SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. 119. Microplate Reader, For Lab. Find regional support. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe VersaMax and SpectraMax 190 read 96-well microplates. The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. com always has the largest. ScanLater Western Blot Detection Cartridge. The VersaMax and SpectraMax 190 read 96-well microplates. For very concentrated samples, use a minimumSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. aliquot size needed for sequencing)We would like to show you a description here but the site won’t allow us. , Tokyo, Japan) was added to each well every 24 h. In brief, 8-well chamber culture plates were coated with a thin layer of 8. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. overnight at room. Sep 19, 2023 11am PT Date Format. 034 g, 0. Each sample has a discrete sample beam and reference. To contact technical support by phone in the U. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. The SpectraMax ® Plus 384 Microplate Reader gives you walk-up convenience for both standard spectrophotometer and microplate reader applications on. View our entire inventory of New Or Used Roadtrek RVs. Table 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. . 119. The temperature in the plate chamber is ambient and isothermal. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard. Description. . Published Dec 16, 2020 | Updated Dec 16, 2020. 9 to 190 ng/µL were prepared in TE buffer and pipetted onto the wells (spots) of the SpectraDrop Micro-Volume. used. After treatment with paramylon or lipopolysaccharide for 24 h, 10 μL of cell counting kit reagents was added to the cells. It measures the optical density (OD) of samples in 96-well 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wella SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The decrease in absorbance due to decomposition of H 2 O 2 was monitored at 240 nm in a SpectraMax 190 microtitre plate-reader. Capable of detecting DNA down to 25 ng per well, this model gives users the same. A. The UV-vis. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method. Molecular Devices LLC 96 well microplate spectrophotometer spectramax 190 96 Well Microplate Spectrophotometer Spectramax 190, supplied by Molecular Devices LLC, used in various techniques. The iron contents were monitored at 562 nm using a microplate spectrophotometer (SPECTRAmax 190, Molecular Devices Corporation, USA). This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. The dichloromethane, methanol, and hexane extracts were eluted four times and the absorbance spectra of the samples was measured at a range of wavelengths from 200 to 750 nm. We assessed the expression of pro- and anti-inflammatory genes expressed by macrophages, including. Spectramax 190 Absorbance Microplate reader, included validation report, control software, 190-850 nm, Fully inspected and validated with Warranty. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). SpectraMax 190 Absorbance Microplate Reader. SpectraMax and VersaMax . Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. According to the manufacturer, the sensitivity of this assay is 5 ng/ml and has less than 1% cross. Introduction. The absorbance was measured using ELISA analytical instruments SpectraMax 190 (Thermo Fisher Scientific, CA, United States) at a wavelength of 450 nm. Temperature uniformity within the microplate may vary depending on the type of microplate used. A four‐parameter logistic function was used to fit the curve and determine unknown concentrations with the software. Flavonoid Compositions Analysis . The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. . (2012). Description 1. Fluorescence intensity was measured using a. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected]®Plus384addstheabilitytoread cuvettes. 0 out of 5 $ 2,499 $ 1,999. No accessories are needed and SoftMax® Pro Software. The VersaMax and SpectraMax 190 read 96-well microplates. Condition:. Add to Cart. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- The VersaMax and SpectraMax 190 read 96-well microplates. 25% HF. Italic and boldface type are used for emphasis. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Upgradeable modules are also available including western blot, cell imaging, and fast. Analysis of pro-inflammatory macrophage phenotypic response to NAM and RSV. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. , China). With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The VersaMax and SpectraMax 190 read 96-well microplates. Perkin Elmer Wallac Victor2 1420 Multilabel Counter波长范围 190-1000 nm 调节步进 1 nm 波长带宽 2 nm 波长精确度 ± 1 nm 波长重复性 ± 0. 3 Figures from 612 Publications Promega. Patient samples were diluted 1:100, except for patients with CML for whom a dilution of 1:300 was used. 2. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression.